Specifications:
Chemical Formula: C43H77N5O14
Molecular Weight: 888.10
CAS: N/A
Purity: >90%
Physical Form: colorless oil
Solubility: DCM, THF, acetonitrile, DMF and DMSO
Storage at: -20 oC, avoid light
Amino-bis-PEG3-TCO is a trifunctional linker containing two TCO moieties and a secondary amine. TCO  will react with tetrazines via inverse electron demand Diels-Alder cycloaddition to form a stable dihydropyridazine linkage. The extremely fast kinetics and selectivity enables the conjugation of two low abundance biopolymers in an aqueous and otherwise complex chemical environment. This bioorthogonal reaction possesses excellent selectivity and biocompatibility such that the complimentary partners can react with each other within richly functionalized biological systems, in some cases, living organisms. Thus, tetrazine-TCO ligation has found numerous applications in fluorescent imaging, drug delivery, PET and SPECT imaging, radionuclide therapy, radiochemistry or drug target identification among several others.
Excellent biocompatibility – tetrazine click reaction occurs efficiently under mild buffer conditions; requires no accessory reagents such as a copper catalyst or reducing agents (e.g. DTT)
High chemoselectivity – tetrazine and TCO groups do not react or interfere with other functional groups found in biological samples but conjugate to one another with high efficiency
Hydrophilic PEG3 Spacer – reduces aggregation, minimizes sterical hindrance, and enhances solubility