Specifications:
Chemical Formula: C34H33N7O3S2
Molecular Weight: 651.81
CAS: N/A
Purity: >95% by HPLC
Physical Form: red solid
Solubility: THF, DCM, DMF and DMSO
Storage at: -20 oC
DBCO-SS-methyltetrazine is a cleavable heterobifunctional linker functionalized with a methyltetrazine moiety for inverse electron demand Diels-Alder cycloaddition reactions and a DBCO moiety for click chemistry. The dibenzocyclooctyne group (DBCO) is thermally stable with high specific reactivity toward azide group through strain-promoted click reaction in the absence of a catalyst. The tetrazine will react with strained alkenes such as trans-cyclooctene, norbornene and cyclopropene to yield a stable dihydropyridazine linkage.
Biocompatible – click reaction occurs efficiently under mild buffer conditions; requires no accessory reagents such as a copper catalyst or reducing agents (e.g. DTT)
Chemoselective – TCO and tetrazine, azide and DBCO groups do not react or interfere with other functional groups found in biological samples but conjugate to one another with high efficiency
Unprecedented kinetics – inverse-electron demand Diels-Alder chemistry is the fastest bioorthogonal ligation available
Cleavable SS bond – can be cleaved with DTT, GSH or TCEP