Specifications:
Chemical Formula: C15H13N5O4
Molecular Weight: 327.29
CAS: 1644644-96-1
Purity: >95% by HPLC
Physical Form: red solid
Solubility: THF, DCM, DMF and DMSO
Storage at: -20 oC
Methyltetrazine NHS is a heterobifunctional linker functionalized with a tetrazine moiety for inverse electron demand Diels-Alder cycloaddition reactions and an activated NHS ester. The tetrazine will react with strained alkenes such as trans-cyclooctene, norbornene and cyclopropene to yield a stable dihydropyridazine linkage.
Biocompatible – click reaction occurs efficiently under mild buffer conditions; requires no accessory reagents such as a copper catalyst or reducing agents (e.g. DTT)
Chemoselective – tetrazines and alkynes do not react or interfere with other functional groups found in biological samples but conjugate to one another with high efficiency
Unprecedented kinetics – inverse-electron demand Diels-Alder chemistry is the fastest bioorthogonal ligation available