Specifications:
Chemical Formula: C48H51N9O8
Molecular Weight: 881.97
CAS: N/A
Purity: >90% by HPLC
Physical Form: red solid
Solubility: THF, DCM, DMF and DMSO
Storage at: -20 oC
Methyltetrazine-PEG4-hydrazone-DBCO is a cleavable heterobifunctional linker functionalized with a methyltetrazine moiety for inverse electron demand Diels-Alder cycloaddition reactions and a DBCO moiety for click chemistry.The dibenzocyclooctyne group (DBCO) is thermally stable with high specific reactivity toward azide group through strain-promoted click reaction in the absence of a catalyst. The tetrazine will react with strained alkenes such as trans-cyclooctene, norbornene and cyclopropene to yield a stable dihydropyridazine linkage.
Biocompatible – click reaction occurs efficiently under mild buffer conditions; requires no accessory reagents such as a copper catalyst or reducing agents (e.g. DTT)
Chemoselective – TCO and tetrazine, azide and DBCO groups do not react or interfere with other functional groups found in biological samples but conjugate to one another with high efficiency
Unprecedented kinetics – inverse-electron demand Diels-Alder chemistry is the fastest bioorthogonal ligation available
Hydrophilic spacer – PEG4 spacer enhances solubility in aqueous buffers
Cleavable hydrazone bond – can be cleaved under acidic condition