Specifications:
Chemical Formula: C85H122N20O28
Molecular Weight: 1872.00
CAS: N/A
Purity: >90%
Physical Form: red oil
Solubility: DCM, THF, acetonitrile, DMF and DMSO
Storage at: -20 oC
NHS-PEG5-tris-PEG3-tetrazine is a heterotetrafunctional linker containing three tetrazine moieties for inverse electron demand Diels-Alder cycloaddition reactions and an NHS activated ester group for amide formation. The tetrazine will react with strained alkenes such as trans-cyclooctene, norbornene and cyclopropene to yield a stable dihydropyridazine linkage. The extremely fast kinetics and selectivity enables the conjugation of two low abundance biopolymers in an aqueous and otherwise complex chemical environment. This bioorthogonal reaction possesses excellent selectivity and biocompatibility such that the complimentary partners can react with each other within richly functionalized biological systems, in some cases, living organisms.
Biocompatible – click reaction occurs efficiently under mild buffer conditions; requires no accessory reagents such as a copper catalyst or reducing agents (e.g. DTT)
Chemoselective – tetrazines and trans-cyclooctene groups do not react or interfere with other functional groups found in biological samples but conjugate to one another with high efficiency
Unprecedented kinetics – inverse-electron demand Diels-Alder chemistry is the fastest bioorthogonal ligation available
Hydrophilic spacer – PEG spacer enhances solubility in aqueous buffers