Tetrazine-SS-Biotin

Specifications:

Chemical Formula: C₂₄H₃₂N₈O₃S₃
Molecular Weight: 576.77
CAS: N/A
Purity: >90%
Physical Form: red oil
Solubility: DCM, acetonitrile, DMF and DMSO
Storage at: -20 oC

Tetrazine-SS-Biotin is a cleavable biotinylation reagent containing a tetrazine moiety for inverse electron demand Diels-Alder cycloaddition reactions. The tetrazine will react with strained alkenes such as trans-cyclooctene, norbornene and cyclopropene to yield a stable dihydropyridazine linkage. The integral disulfide bond enables the release of the biotin group from the labeled molecule using DTT or other reducing agents

Biocompatible – click reaction occurs efficiently under mild buffer conditions; requires no accessory reagents such as a copper catalyst or reducing agents (e.g. DTT)

Chemoselective – tetrazines and trans-cyclooctene groups do not react or interfere with other functional groups found in biological samples but conjugate to one another with high efficiency

Unprecedented kinetics – inverse-electron demand Diels-Alder chemistry is the fastest bioorthogonal ligation available

Cleavable SS bond – can be cleaved with DTT, GSH or TCEP